SF20/MYDGF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98493

Novus Biologicals, part of Bio-Techne
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NBP1-98493
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SF20/MYDGF Antibodies »

Product Summary for SF20/MYDGF Antibody

Immunogen

The immunogen for this antibody is a synthetic peptide, aa 67-116, from the middle region of SF20/MYDGF protein (NP_061980). Peptide sequence YASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAE. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SF20/MYDGF Antibody

Western Blot: SF20/MYDGF Antibody [NBP1-98493]

Western Blot: SF20/MYDGF Antibody [NBP1-98493]

Western Blot: SF20/MYDGF Antibody [NBP1-98493] - Antibody Dilution: 1.0ug/ml Sample Tissue: Human Placenta.
Immunohistochemistry-Paraffin: SF20/MYDGF Antibody [NBP1-98493]

Immunohistochemistry-Paraffin: SF20/MYDGF Antibody [NBP1-98493]

Immunohistochemistry-Paraffin: SF20/MYDGF Antibody [NBP1-98493] - Antibody Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Cytoplasm in hepatocytes, moderate tissue distribution, resemble Golgi structures

Applications for SF20/MYDGF Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human)

Gene Symbol

MYDGF

UniProt

Q969H8

Additional SF20/MYDGF Products

Product Documents for SF20/MYDGF Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF20/MYDGF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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