SF3A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79843

Novus Biologicals, part of Bio-Techne
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NB100-79843
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SF3A1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human splicing factor 3a, subunit 1, 120kDa using the numbering given in entry NP_005868.1 (GeneID 10291).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF3A1 Antibody

Western Blot: SF3A1 Antibody [NB100-79843]

Western Blot: SF3A1 Antibody [NB100-79843]

Western Blot: SF3A1 Antibody [NB100-79843] - Detection of Human SF3a120/SAP114 on HeLa whole cell lysate using NB100-79843. SF3a120/SAP114 was also IPed by rabbit anti-SF3a120/SAP114 antibodies NB100-79844, NB100-79845, and NB100-79846.
Immunohistochemistry: SF3A1 Antibody [NB100-79843]

Immunohistochemistry: SF3A1 Antibody [NB100-79843]

Immunohistochemistry: SF3A1 Antibody [NB100-79843] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- SF3a120/SAP114 used at a dilution of 1:200 (1 ug/ml). Detection: DAB

Applications for SF3A1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID 28504715).

Reviewed Applications

Read 1 review rated 4 using NB100-79843 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SF3A1

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

pre-mRNA processing 21, pre-mRNA splicing factor SF3a (120 kDa subunit), Prp21, PRPF21, SAP 114, SAP114splicing factor 3a, subunit 1, 120kD, SF3A120, spliceosome associated protein 114, Spliceosome-associated protein 114, splicing factor 3 subunit 1, splicing factor 3A subunit 1, splicing factor 3a, subunit 1, 120kDa

Entrez Gene IDs

10291 (Human)

Gene Symbol

SF3A1

UniProt

Q15459 (Human)

Additional SF3A1 Products

Product Documents for SF3A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF3A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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