SF3A2 Antibody (3B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00008175-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SF3A2 Antibodies »

Product Specifications

Immunogen

SF3A2 (NP_009096, 112 a.a. ~ 216 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IGRPGYKVTKQRDSEMGQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEK

Specificity

SF3A2 (3B6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SF3A2 Antibody (3B6)

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01]

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01] - SF3A2 expression in NIH/3T3 ( Cat # L018V1 ).

Immunocytochemistry/ Immunofluorescence: SF3A2 Antibody (3B6) [H00008175-M01]

Immunocytochemistry/Immunofluorescence: SF3A2 Antibody (3B6) [H00008175-M01] - Analysis of monoclonal antibody to SF3A2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01]

Western Blot: SF3A2 Antibody (3B6) [H00008175-M01] - SF3A2 expression in PC-12 ( Cat # L012V1 ).

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Applications for SF3A2 Antibody (3B6)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IP usage reported in scientific literature (PMID: 22672905).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SF3A2

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

Alternate Names

pre-mRNA splicing factor SF3A, subunit 2, PRP11, PRPF11, SAP 62, SAP62Prp11, SF3a66splicing factor 3a, subunit 2, 66kD, spliceosome associated protein 62, Spliceosome-associated protein 62, splicing factor 3A subunit 2, splicing factor 3a, subunit 2, 66kDa

Entrez Gene IDs

8175 (Human)

Gene Symbol

SF3A2

OMIM

600796 (Human)

UniProt

NP_009096 (Human)

Additional SF3A2 Products

Product Documents for SF3A2 Antibody (3B6)

Product Datasheets

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COA

SDS

Product Specific Notices for SF3A2 Antibody (3B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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