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SF3A3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94076

Novus Biologicals, part of Bio-Techne
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NBP2-94076-0.02ml
NBP2-94076-0.1ml

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3 (NP_006793.1). SLESLDTSLFAKNPKSKGTKRDTERNKDIAFLEAQIYEYVEILGEQRHLTHENVQRKQARTGEEREEEEEEQISESESEDEENEIIYNPKNLPLGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKAFQRHFAEWRHAHGMRCLGIPNTAHFANVTQIEDAVSLWAKLKLQKASERWQPDTEEEYEDSSGNVVNKKTYEDLKRQGLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SF3A3 Antibody - Azide and BSA Free

Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]

Western Blot: SF3A3 AntibodyAzide and BSA Free [NBP2-94076]

Western Blot: SF3A3 Antibody [NBP2-94076] - Western blot analysis of extracts of various cell lines, using SF3A3 antibody (NBP2-94076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]

Immunocytochemistry/ Immunofluorescence: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]

Immunocytochemistry/Immunofluorescence: SF3A3 Antibody [NBP2-94076] - Analysis of U2OS cells using SF3A3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]

Immunohistochemistry-Paraffin: SF3A3 Antibody - Azide and BSA Free [NBP2-94076]

Immunohistochemistry-Paraffin: SF3A3 Antibody [NBP2-94076] - Immunohistochemistry of paraffin-embedded mouse kidney using SF3A3 Rabbit pAb (NBP2-94076) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for SF3A3 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SF3A3

The SF3A3 gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicin

Alternate Names

PRP9, PRPF9, SAP 61, SAP61splicing factor 3a, subunit 3, 60kD, SF3a60pre-mRNA splicing factor SF3a (60kD), spliceosome associated protein 61, Spliceosome-associated protein 61, splicing factor 3A subunit 3, splicing factor 3a, subunit 3, 60kDa

Gene Symbol

SF3A3

Additional SF3A3 Products

Product Documents for SF3A3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SF3A3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov