SF3B4 Antibody (1B8)

Novus Biologicals, part of Bio-Techne | Catalog # H00010262-M03

Novus Biologicals, part of Bio-Techne
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H00010262-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SF3B4 Antibodies »

Product Specifications

Immunogen

SF3B4 (NP_005841.1, 13 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSA

Specificity

SF3B4 - splicing factor 3b, subunit 4, 49kDa (1B8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SF3B4 Antibody (1B8)

Western Blot: SF3B4 Antibody (1B8) [H00010262-M03]

Western Blot: SF3B4 Antibody (1B8) [H00010262-M03]

Western Blot: SF3B4 Antibody (1B8) [H00010262-M03] - Analysis of SF3B4 expression in transfected 293T cell line by SF3B4 monoclonal antibody (M03), clone 1B8. Lane 1: SF3B4 transfected lysatE (44.4 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: SF3B4 Antibody (1B8) [H00010262-M03] - Detection limit for recombinant GST tagged SF3B4 is 0.03 ng/ml as a capture antibody.

Applications for SF3B4 Antibody (1B8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SF3B4

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq]

Alternate Names

Hsh49, pre-mRNA splicing factor SF3b 49 kDa subunit, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49MGC10828, SF3B49, SF3b50, spliceosomal protein, spliceosome-associated protein (U2 snRNP), Spliceosome-associated protein 49, splicing factor 3B subunit 4, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa

Entrez Gene IDs

10262 (Human)

Gene Symbol

SF3B4

UniProt

NP_005841 (Human)

Additional SF3B4 Products

Product Documents for SF3B4 Antibody (1B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SF3B4 Antibody (1B8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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