SF3B4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1076

Novus Biologicals, part of Bio-Techne
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NB100-1076
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SF3B4 Antibodies »

Product Summary for SF3B4 Antibody

Immunogen

Peptide with sequence AAGPISERNQDAT-C corresponding to N-Terminus according to NP_005841.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Localization

Nuclear

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SF3B4 Antibody

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076] - Western blot analysis of SF3B4 in Hela lysate using NB100-1076 at 0.1 ug/ml.
Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076]

Western Blot: SF3B4 Antibody [NB100-1076] - staining (0.1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for SF3B4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human HeLa lysates (calculated MW of 44.4 kDa band according to NP005841.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SF3B4

The SF3B4 gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

Alternate Names

Hsh49, pre-mRNA splicing factor SF3b 49 kDa subunit, Pre-mRNA-splicing factor SF3b 49 kDa subunit, SAP 49, SAP49MGC10828, SF3B49, SF3b50, spliceosomal protein, spliceosome-associated protein (U2 snRNP), Spliceosome-associated protein 49, splicing factor 3B subunit 4, splicing factor 3b, subunit 4, 49kD, splicing factor 3b, subunit 4, 49kDa

Entrez Gene IDs

10262 (Human); 107701 (Mouse)

Gene Symbol

SF3B4

UniProt

NP_005841. (Human)

Additional SF3B4 Products

Product Documents for SF3B4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SF3B4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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