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SF4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88000

Novus Biologicals, part of Bio-Techne
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NBP1-88000
NBP1-88000-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GKANRWFGVAPPKSGKMNMNILHQEELIAQKKREIEAKMEQKAKQNQVASPQPPHPGEITNAHNSSCISNKFANDGSFLQQFLKLQKAQTSTDAPTSAPSAPPSTPT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF4 Antibody

Western Blot: SF4 Antibody [NBP1-88000]

Western Blot: SF4 Antibody [NBP1-88000]

Western Blot: SF4 Antibody [NBP1-88000] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: SF4 Antibody [NBP1-88000]

Immunocytochemistry/ Immunofluorescence: SF4 Antibody [NBP1-88000]

Immunocytochemistry/Immunofluorescence: SF4 Antibody [NBP1-88000] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SF4 Antibody [NBP1-88000]

Immunohistochemistry-Paraffin: SF4 Antibody [NBP1-88000]

Immunohistochemistry-Paraffin: SF4 Antibody [NBP1-88000] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.

Applications for SF4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SF4

SF4 is a member of the SURP family of splicing factors.(supplied by OMIM)

Alternate Names

DKFZp434E2216, F23858, SF4RNA-binding protein RBP, Splicing factor 4RBP, SURP and G patch domain containing 1, SURP and G-patch domain-containing protein 1

Gene Symbol

SUGP1

Additional SF4 Products

Product Documents for SF4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SF4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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