SFPQ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61043

Novus Biologicals, part of Bio-Techne
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NB100-61043
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SFPQ Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutaminerich) using the numbering given in entry NP_005057.1 (GeneID 6421).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFPQ Antibody

Western Blot: SFPQ Antibody [NB100-61043]

Western Blot: SFPQ Antibody [NB100-61043]

Western Blot: SFPQ Antibody [NB100-61043] - Detection of Human PSF/SFPQ on HeLa whole cell lysate (5,15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-61043. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies NB100-61044 and NB100-61045.
Immunohistochemistry: SFPQ Antibody [NB100-61043]

Immunohistochemistry: SFPQ Antibody [NB100-61043]

Immunohistochemistry: SFPQ Antibody [NB100-61043] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PSF/SFPQ used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for SFPQ Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SFPQ

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination.

Alternate Names

100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, hPOMp100, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, Polypyrimidine tract-binding protein-associated-splicing factor, PSFPOMP100, PTB-associated splicing factor, PTB-associated-splicing factor, splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated), splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated), splicing factor proline/glutamine-rich, splicing factor, proline- and glutamine-rich

Entrez Gene IDs

6421 (Human)

Gene Symbol

SFPQ

UniProt

P23246 (Human)

Additional SFPQ Products

Product Documents for SFPQ Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SFPQ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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