SFPQ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61045

Novus Biologicals, part of Bio-Techne
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NB100-61045
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all SFPQ Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 657 and 707 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutamine-rich) using the numbering given in entry NP_005057.1 (GeneID 6421).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFPQ Antibody

Western Blot: SFPQ Antibody [NB100-61045]

Western Blot: SFPQ Antibody [NB100-61045]

Western Blot: SFPQ Antibody [NB100-61045] - Detection of Human and Mouse SFPQ on HeLa whole cell lysate using NB100-61045. SFPQ was also immunoprecipitated by rabbit anti-SFPQ antibodies NB100-61043 and NB100-61044.
Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045]

Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045]

Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045] - Sample: FFPE section of human prostate carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti- PSF/SFPQ used at a dilution of 1:500 (0.4 ug/ml). Detection: DAB

Applications for SFPQ Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in (PMID: 23644382).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SFPQ

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination.

Alternate Names

100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, hPOMp100, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, Polypyrimidine tract-binding protein-associated-splicing factor, PSFPOMP100, PTB-associated splicing factor, PTB-associated-splicing factor, splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated), splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated), splicing factor proline/glutamine-rich, splicing factor, proline- and glutamine-rich

Entrez Gene IDs

6421 (Human); 71514 (Mouse)

Gene Symbol

SFPQ

UniProt

P23246 (Human)

Additional SFPQ Products

Product Documents for SFPQ Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SFPQ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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