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sFRP-3/FRZB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-79552

Novus Biologicals, part of Bio-Techne
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NBP1-79552

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptide directed towards the middle region of human FRZBThe immunogen for this antibody is FRZB. Peptide sequence VVEVKEILKSSLVNIPRDTVNLYTSSGCLCPPLNVNEEYIIMGYEDEERS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for sFRP-3/FRZB Antibody

Western Blot: sFRP-3/FRZB Antibody [NBP1-79552]

Western Blot: sFRP-3/FRZB Antibody [NBP1-79552]

Western Blot: sFRP-3/FRZB Antibody [NBP1-79552] - Titration: 0.4 ug/ml Positive Control: human synovium patient.
Immunohistochemistry-Paraffin: sFRP-3/FRZB Antibody [NBP1-79552]

Immunohistochemistry-Paraffin: sFRP-3/FRZB Antibody [NBP1-79552]

Immunohistochemistry-Paraffin: sFRP-3/FRZB Antibody [NBP1-79552] - Human spleen cell lysate tissue at an antibody concentration of 5 ug/ml.
Western Blot: sFRP-3/FRZB Antibody [NBP1-79552]

Western Blot: sFRP-3/FRZB Antibody [NBP1-79552]

Western Blot: sFRP-3/FRZB Antibody [NBP1-79552] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.

Applications for sFRP-3/FRZB Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: sFRP-3

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interactionwith Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appearsto be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bonedevelopment

Long Name

Secreted Frizzled Related Protein 3

Alternate Names

Fritz, FRZB, Frzb1, sFRP3

Gene Symbol

FRZB

Additional sFRP-3 Products

Product Documents for sFRP-3/FRZB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for sFRP-3/FRZB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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