Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SFRS18 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 to 250 of human splicing factor, arginine/serine-rich 18 (splicing factor, arginine/serine-rich 130) using the numbering given in entry NP_056306.1 (GeneID 25957).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SFRS18 Antibody

Immunoprecipitation: SFRS18 Antibody [NB100-79851]

Immunoprecipitation: SFRS18 Antibody [NB100-79851]

Immunoprecipitation: SFRS18 Antibody [NB100-79851] - Detection of Human SFRS18/SRrp130 by Western Blot of Immunoprecipitates. For blSamples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SFRS18/SRrp130 antibody NB100-79851 used for IP at 3 mcg/mg lysate. SFRS18/SRrp130 was also immunoprecipitated by rabbit anti-SFRS18/SRrp130 antibody NB100-79852, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SFRS18 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SFRS18

SFRS18/SRrp130 (splicing factor arginine/serine-rich 18) is a putative alternative splicing factor. It was identified as a factor that interacts with pinin (Pnn/DRS/memA), a cell-adhesion and nuclear protein of corneal epithelial cells. SFRS18/SRrp130 bears multiple arginine/serine-rich repeats found among the SR-related family of pre-mRNA processing factors. SFRS18/SRrp130 and pinin co-localize and are distributed as nuclear specks that correspond to the pre-splicing machinery.

Alternate Names

bA98I9.2, C6orf111, chromosome 6 open reading frame 111, DKFZp564B0769, FLJ14752, FLJ14992, FLJ90147, HSPC306, MGC104269, RP11-98I9.2, Serine/arginine-rich-splicing regulatory protein 130, serine-arginine-rich-splicing regulatory protein 130, Splicing factor, arginine/serine-rich 130, splicing factor, arginine/serine-rich 18, SR rich protein, SR-related protein, SR-rich protein, SRRP130, SRrp130FLJ14853

Entrez Gene IDs

25957 (Human)

Gene Symbol

PNISR

UniProt

NP_056306.1 (Human)

Additional SFRS18 Products

Product Documents for SFRS18 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SFRS18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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