SgK071 Antibody (2B5)
Novus Biologicals, part of Bio-Techne | Catalog # H00169436-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2B5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
C9orf96 (NP_714921, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLGPGSNRRRPTQGERGPGSPGEPMEKYQVLYQLNPGALGVNLVVEEMETKVKHVIKQVECMDDHYASQALEELMPLLKLRHAHISVYQELFITWNGEI
Reactivity Notes
Human. Other species not tested.
Specificity
C9orf96 - chromosome 9 open reading frame 96
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for SgK071 Antibody (2B5)
![Western Blot: SgK071 Antibody (2B5) [H00169436-M03]](https://resources.bio-techne.com/images/products/SgK071-Antibody-2B5-Western-Blot-H00169436-M03-img0004.jpg "Western Blot: SgK071 Antibody (2B5) [H00169436-M03]")
Western Blot: SgK071 Antibody (2B5) [H00169436-M03]
Western Blot: SgK071 Antibody (2B5) [H00169436-M03] - Analysis of C9orf96 expression in transfected 293T cell line by C9orf96 monoclonal antibody (M03), clone 2B5. Lane 1: C9orf96 transfected lysatE (75.5 KDa). Lane 2: Non-transfected lysate.![Western Blot: SgK071 Antibody (2B5) [H00169436-M03]](https://resources.bio-techne.com/images/products/SgK071-Antibody-2B5-Western-Blot-H00169436-M03-img0002.jpg "Western Blot: SgK071 Antibody (2B5) [H00169436-M03]")
Western Blot: SgK071 Antibody (2B5) [H00169436-M03]
Western Blot: SgK071 Antibody (2B5) [H00169436-M03] - Western blot analysis of C9orf96 over-expressed 293 cell line. Lane 2 cotransfected with C9orf96 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00169436-M03. GAPDH(36.1 kDa) used as loading control.![ELISA: SgK071 Antibody (2B5) [H00169436-M03]](https://resources.bio-techne.com/images/products/SgK071-Antibody-2B5-ELISA-H00169436-M03-img0003.jpg "ELISA: SgK071 Antibody (2B5) [H00169436-M03]")
ELISA: SgK071 Antibody (2B5) [H00169436-M03]
ELISA: SgK071 Antibody (2B5) [H00169436-M03] - Detection limit for recombinant GST tagged C9orf96 is approximately 0.03ng/ml as a capture antibody.Applications for SgK071 Antibody (2B5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SgK071
Alternate Names
chromosome 9 open reading frame 96, MGC43306, protein kinase-like protein SgK071, RP11-244N20.8, SgK071, Sk521, Sugen kinase 071
Entrez Gene IDs
169436 (Human)
Gene Symbol
C9ORF96
UniProt
Additional SgK071 Products
Product Documents for SgK071 Antibody (2B5)
Product Specific Notices for SgK071 Antibody (2B5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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