SH2B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13305

Novus Biologicals, part of Bio-Techne
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NBP2-13305
NBP2-13305-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SH2B2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RDKWTRRLRLSRTLAAKVELVDIQREGALRFMVADD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SH2B2 Antibody

Western Blot: SH2B2 Antibody [NBP2-13305]

Western Blot: SH2B2 Antibody [NBP2-13305]

Western Blot: SH2B2 Antibody [NBP2-13305] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: SH2B2 Antibody [NBP2-13305]

Immunocytochemistry/ Immunofluorescence: SH2B2 Antibody [NBP2-13305]

Immunocytochemistry/Immunofluorescence: SH2B2 Antibody [NBP2-13305] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: SH2B2 Antibody [NBP2-13305]

Immunohistochemistry-Paraffin: SH2B2 Antibody [NBP2-13305]

Immunohistochemistry-Paraffin: SH2B2 Antibody [NBP2-13305] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.

Applications for SH2B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SH2B2

APS (adapter protein with Pleckstrin homology and Src homology 2 domains) is a member of the Lnk family, an adapter protein that is involved in B cell signaling, insulin signaling and cytoskeletal reorganization. A PH and an SH2 domain containing adapter protein that links activated tyrosine kinases to signaling pathways. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK STAT pathway in collaboration with cCbl.

Alternate Names

adaptor protein with pleckstrin homology and src homology 2 domains, APSAdapter protein with pleckstrin homology and Src homology 2 domains, SH2 and PH domain-containing adapter protein APS, SH2B adapter protein 2, SH2B adaptor protein 2

Gene Symbol

SH2B2

Additional SH2B2 Products

Product Documents for SH2B2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SH2B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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