SH3BGRL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33609

Novus Biologicals, part of Bio-Techne
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NBP2-33609
NBP2-33609-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SH3BGRL Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FNESQYRGDYDAFFEARENNAVYAFLGLTAPPGSKEAEVQAKQQA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SH3BGRL Antibody

Western Blot: SH3BGRL Antibody [NBP2-33609]

Western Blot: SH3BGRL Antibody [NBP2-33609]

Western Blot: SH3BGRL Antibody [NBP2-33609] - Analysis in human cell lines SK-MEL-30 and A-431 using anti-SH3BGRL antibody. Corresponding SH3BGRL RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: SH3BGRL Antibody [NBP2-33609]

Immunocytochemistry/ Immunofluorescence: SH3BGRL Antibody [NBP2-33609]

Immunocytochemistry/Immunofluorescence: SH3BGRL Antibody [NBP2-33609] - Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.
Immunohistochemistry-Paraffin: SH3BGRL Antibody [NBP2-33609]

Immunohistochemistry-Paraffin: SH3BGRL Antibody [NBP2-33609]

Immunohistochemistry-Paraffin: SH3BGRL Antibody [NBP2-33609] - Staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.
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Applications for SH3BGRL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SH3BGRL

SH3BGRL is a gene that codes for a protein measuring 114 amino acids in length and approximately 13 kDa in weight that functions as a SH3 domain-binding glutamic acid-rich-like protein. Current studies are being done on diseases and disorders linked to this gene including endocarditis and obesity. SH3BGRL has also been shown to have interactions with EGFR, ATG5, ERBB2, IL7R, and TPT1 in pathways such as the EGFR1 signaling pathway.

Alternate Names

MGC117402, SH3 domain binding glutamic acid-rich protein like, SH3 domain-binding glutamic acid-rich-like protein, SH3BGR, SH3-binding domain glutamic acid-rich protein like

Gene Symbol

SH3BGRL

UniProt

O75368 (Human)

Additional SH3BGRL Products

Product Documents for SH3BGRL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SH3BGRL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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