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SH3BP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1398

Novus Biologicals, part of Bio-Techne
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NB100-1398

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-PQPRPRSLASETN corresponding to C-Terminus according to NP_061830.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SH3BP1 Antibody

Western Blot: SH3BP1 Antibody [NB100-1398]

Western Blot: SH3BP1 Antibody [NB100-1398]

Western Blot: SH3BP1 Antibody [NB100-1398] - SH3BP1 in Human Brain lysate using NB100-1398 at 1 ug/ ml.
Immunohistochemistry-Paraffin: SH3BP1 Antibody [NB100-1398]

Immunohistochemistry-Paraffin: SH3BP1 Antibody [NB100-1398]

Immunohistochemistry-Paraffin: SH3BP1 Antibody [NB100-1398] - NB100-1398 (0.3ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for SH3BP1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3 ug/ml

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
ICC successful usage in Caco-2 cells is reported in scientific literature (Elbediwy et al, J.Biol. Chem, 2012). WB: Approx. 70 kDa band observed in human lung and kidney lysates (calculated MW of 75.7 kDa band according to NP_061830.3). IHC-P: Human kidney stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SH3BP1

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to c-Abl proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac (By similarity).

Alternate Names

3BP-1, ARHGAP43, SH3 domain binding protein 1, SH3 domain-binding protein 1, SH3-domain binding protein 1

Entrez Gene IDs

23616 (Human)

Gene Symbol

SH3BP1

Additional SH3BP1 Products

Product Documents for SH3BP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SH3BP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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