SH3MD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54972

Novus Biologicals, part of Bio-Techne
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NBP1-54972
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SH3MD2 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to SH3RF1(SH3 domain containing ring finger 1) The peptide sequence was selected from the middle region of SH3RF1. Peptide sequence LLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SH3MD2 Antibody

Western Blot: SH3MD2 Antibody [NBP1-54972]

Western Blot: SH3MD2 Antibody [NBP1-54972]

Western Blot: SH3MD2 Antibody [NBP1-54972] - Titration: 0.2-1 ug/ml, Positive Control: DU145 cell lysate.

Applications for SH3MD2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SH3MD2

SH3RF1 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3RF1 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane.This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Alternate Names

EC 6.3.2, EC 6.3.2.-, KIAA1494SH3 multiple domains protein 2, Plenty of SH3s, POSHFLJ21602, Protein POSH, putative E3 ubiquitin-protein ligase SH3RF1, RING finger protein 142, RNF142plenty of SH3 domains, SH3 domain containing ring finger 1, SH3 domain-containing RING finger protein 1, SH3 multiple domains 2, SH3MD2

Gene Symbol

SH3RF1

UniProt

Q7Z6J0 (Human)

Additional SH3MD2 Products

Product Documents for SH3MD2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SH3MD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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