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SH3MD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20347

Novus Biologicals, part of Bio-Techne
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NBP2-20347

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SH3MD2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (80%).

Localization

Cytoplasm; perinuclear region (By similarity); Cell projection; lamellipodium; Golgi apparatus; trans-Golgi network

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SH3MD2 Antibody

Western Blot: SH3MD2 Antibody [NBP2-20347]

Western Blot: SH3MD2 Antibody [NBP2-20347]

Western Blot: SH3MD2 Antibody [NBP2-20347] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SH3MD2 Antibody [NBP2-20347]

Immunohistochemistry-Paraffin: SH3MD2 Antibody [NBP2-20347]

Immunohistochemistry-Paraffin: SH3MD2 Antibody [NBP2-20347] - Immunohistochemical analysis of paraffin-embedded Normal Colon, using antibody at 1:250 dilution.

Applications for SH3MD2 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SH3MD2

This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq]

Alternate Names

EC 6.3.2, EC 6.3.2.-, KIAA1494SH3 multiple domains protein 2, Plenty of SH3s, POSHFLJ21602, Protein POSH, putative E3 ubiquitin-protein ligase SH3RF1, RING finger protein 142, RNF142plenty of SH3 domains, SH3 domain containing ring finger 1, SH3 domain-containing RING finger protein 1, SH3 multiple domains 2, SH3MD2

Gene Symbol

SH3RF1

UniProt

Additional SH3MD2 Products

Product Documents for SH3MD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SH3MD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.