SHARPIN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04116
![Immunoprecipitation: SHARPIN Antibody [NBP2-04116]](https://resources.bio-techne.com/images/products/SHARPIN-Antibody-Immunoprecipitation-NBP2-04116-img0001.jpg "Immunoprecipitation: SHARPIN Antibody [NBP2-04116]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human Shank-Associated RH Domain-Interacting Protein using the numbering given in entry NP_112236.3 (GeneID 81858).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID:34755089).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHARPIN Antibody
Immunoprecipitation: SHARPIN Antibody [NBP2-04116]
Immunoprecipitation: SHARPIN Antibody [NBP2-04116] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody NBP2-04116 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for SHARPIN Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reactivity reported in scientific literature (PMID:34755089).
Immunohistochemistry
Reactivity reported in scientific literature (PMID:34755089).
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SHARPIN
Long Name
SHANK-associated RH Domain Interactor
Alternate Names
SIPL1
Gene Symbol
SHARPIN
UniProt
Additional SHARPIN Products
Product Documents for SHARPIN Antibody
Product Specific Notices for SHARPIN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...