SHIP Antibody (2F8U0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16226
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2F8U0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1084-1178 of human SHIP1 (Q92835). RSFTCSSSAEGRAAGGDKSQGKPKTPVSSQAPVPAKRPIKPSRSEINQQTPPTPTPRPPLPVKSPAVLHLQHSKGRDYRDNTELPHHGKHRPEEG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHIP Antibody (2F8U0)
Western Blot: SHIP Antibody (2F8U0) [NBP3-16226]
Western Blot: SHIP Antibody (2F8U0) [NBP3-16226] - Western blot analysis of extracts of various cell lines, using SHIP1 Rabbit mAb (NBP3-16226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry: SHIP Antibody (2F8U0) [NBP3-16226]
Immunohistochemistry: SHIP Antibody (2F8U0) [NBP3-16226] - Immunofluorescence analysis of mouse spleen using SHIP1 Rabbit mAb (NBP3-16226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: SHIP Antibody (2F8U0) [NBP3-16226]
Immunohistochemistry-Paraffin: SHIP Antibody (2F8U0) [NBP3-16226] - Immunohistochemistry of paraffin-embedded human spleen using SHIP1 Rabbit mAb (NBP3-16226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for SHIP Antibody (2F8U0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SHIP
Long Name
SH2-containing Inositol Phosphatase
Alternate Names
INPP5D
Gene Symbol
INPP5D
Additional SHIP Products
Product Documents for SHIP Antibody (2F8U0)
Product Specific Notices for SHIP Antibody (2F8U0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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