Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human inositol polyphosphate phosphatase-like 1 using the numbering given in entry NP_001558.2 (GeneID 3636).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHIP2/INPPL1 Antibody

Western Blot: SHIP2/INPPL1 Antibody [NB100-1592]

Western Blot: SHIP2/INPPL1 Antibody [NB100-1592]

Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592]

Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592]

Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592] - Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592]

Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592]

Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 (lot NB100-1592-2) used for IP at 6 ug per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (NB100-1592-1) and another rabbit anti-INPPL2/SHIP2 antibody. For blotting immunoprecipitated INPPL2/SHIP2, NB100-1592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SHIP2/INPPL1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:2000-1:20000
Application Notes
Epitope retrieval with Citrate buffer pH 6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SHIP2

INPPL1 (Inositol Polyphosphate Phosphatase-Like Protein 1) is a phosphatidylinositol phosphatase that negatively regulates phosphoinositide 3-kinase (PI3K) pathways related to numerous cell activities. INPPL1 plays a role in PI3K-dependent insulin, macrophage colony-stimulating factor, and EGF signaling. INPPL1 is also involved in actin cytoskeletal remodeling, actin dynamics, and neuritogenesis. INPPL1 interacts with SHC1 (src-homology domain) to mediate EGFR signaling. Alternate names for INPPL1 include SH2 domain-containing inositol-5'phosphatase 2, SHIP-2, INPPL-1, and protein 51C.

Long Name

SH2-containing Inositol-5'-Phosphatase 2

Alternate Names

INPPL1

Entrez Gene IDs

3636 (Human)

Gene Symbol

INPPL1

UniProt

NP_001558.2 (Human)

Additional SHIP2 Products

Product Documents for SHIP2/INPPL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHIP2/INPPL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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