Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AT26E5

Format

BSA Free

Concentration

1 mg/ml

View all SHMT1 Antibodies »

Product Summary for SHMT1 Antibody (AT26E5) - BSA Free

Immunogen

Recombinant human SHMT1 (1-483aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SHMT1 Antibody (AT26E5) - BSA Free

Western Blot: SHMT1 Antibody (AT26E5)BSA Free [NBP2-59437]

Western Blot: SHMT1 Antibody (AT26E5)BSA Free [NBP2-59437]

Western Blot: SHMT1 Antibody (AT26E5) [NBP2-59437] - Lane 1: A549 cell lysates, Lane 2: HeLa cell lysates, Lane 3: NIH-3T3 cell lysates, Lane 4: LnCaP cell lysates
Immunocytochemistry/ Immunofluorescence: SHMT1 Antibody (AT26E5) - BSA Free [NBP2-59437]

Immunocytochemistry/ Immunofluorescence: SHMT1 Antibody (AT26E5) - BSA Free [NBP2-59437]

Immunocytochemistry/Immunofluorescence: SHMT1 Antibody (AT26E5) [NBP2-59437] - Analysis of SHMT1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SHMT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for SHMT1 Antibody (AT26E5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHMT1

SHMT1 encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Long Name

Serine hydroxymethyltransferase, cytosolic

Alternate Names

EC 2.1.2.1, Serine methylase, SHMT

Gene Symbol

SHMT1

Additional SHMT1 Products

Product Documents for SHMT1 Antibody (AT26E5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SHMT1 Antibody (AT26E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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