SHMT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85437

Novus Biologicals, part of Bio-Techne
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NBP1-85437
NBP1-85437-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SHMT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHMT1 Antibody

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP1-85437]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP1-85437]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP1-85437] - Staining in human kidney and skeletal muscle tissues using NBP1-85437 antibody. Corresponding SHMT1 RNA-seq data are presented for the same tissues.
Western Blot: SHMT1 Antibody [NBP1-85437]

Western Blot: SHMT1 Antibody [NBP1-85437]

Western Blot: SHMT1 Antibody [NBP1-85437] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: SHMT1 Antibody [NBP1-85437]

Immunocytochemistry/ Immunofluorescence: SHMT1 Antibody [NBP1-85437]

Immunocytochemistry/Immunofluorescence: SHMT1 Antibody [NBP1-85437] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.
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Applications for SHMT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25 - 2 ug/mL

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-85437 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2), 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHMT1

SHMT1 encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Long Name

Serine hydroxymethyltransferase, cytosolic

Alternate Names

EC 2.1.2.1, Serine methylase, SHMT

Gene Symbol

SHMT1

Additional SHMT1 Products

Product Documents for SHMT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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