SHP-1 [p Ser591] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23399

Novus Biologicals, part of Bio-Techne
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NBP3-23399
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-1 Antibodies »

Product Summary for SHP-1 [p Ser591] Antibody

Immunogen

Phospho-SHP-1 (Ser-591) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 591 in human SHP-1. The sequence is highly conserved in rat and mouse SHP-1. [UniProt# P29350]

Modification

p Ser591

Specificity

This antibody was cross-adsorbed to a non-specific phospho-serine peptide then affinity-purified using phospho-SHP-1 (Ser-591) peptide. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SHP-1 [p Ser591] Antibody

SHP-1 [p Ser591] Antibody

Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] -

Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] - Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP-1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP-1 (Ser-591) antibody (lanes 3-6). The SHP-1 (Ser-591) antibody was used in the presence of phospho-SHP-1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).

Applications for SHP-1 [p Ser591] Antibody

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SHP-1

The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 [p Ser591] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SHP-1 [p Ser591] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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