Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI7B5

Concentration

1 mg/ml

View all SHP/NR0B2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NR0B2 (NP_068804) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5)

Western Blot: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Western Blot: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Western Blot: SHP/NR0B2/Nuclear Receptor SHP Antibody (7B5) [NBP2-46522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B2.
Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (7B5) [NBP2-46522] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5) [NBP2-46522]

Immunohistochemistry: SHP/NR0B2/Nuclear Receptor SHP Antibody (7B5) [NBP2-46522] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0) .
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Applications for SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP/NR0B2

SHP (short heterodimer partner) is a bile acid-dependent orphan NR0 Knirps-Like receptor with dimerization and ligand-binding domains but not the conventional DNA-binding domain. SHP has been shown to inhibit the transcriptional activity of other nuclear receptors, including thyroid hormone receptor, constitutive androstane receptor, and retinoic acid receptors. SHP affects hepatic cholesterol catabolism based on a two-step mechanism dependent on both coactivator competition and direct transcriptional repression by mediating the repression of CYP7A1, the rate-limiting enzyme for bile acid synthesis. Mutations in the SHP gene contribute to increased body weight, indicating a possible role of SHP in the development of early-onset diabetes (maturity-onset diabetes of the young, MODY). Loss of SHP in mice caused abnormal accumulation and increased synthesis of bile acids due to derepression of rate-limiting CYP7A1 and CYP8B1 hydroxylase enzymes in the biosynthetic pathway. SHP expression has been documented in human liver, adrenal, spleen, and pancreas. ESTs have been isolated from cancerous human kidney, lung, and stomach, as well as normal human heart, kidney, liver, and liver/spleen. Caution: The nuclear receptor SHP should not be confused with the tyrosine phosphatases SHP-1 and SHP-2.

Long Name

Small Heterodimer Partner

Alternate Names

NR0B2

Gene Symbol

NR0B2

Additional SHP/NR0B2 Products

Product Documents for SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP/NR0B2/Nuclear Receptor SHP Antibody (OTI7B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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