SHPRH Antibody (OTI5G8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74180
![Western Blot: SHPRH Antibody (OTI5G8)Azide and BSA Free [NBP2-74180]](https://resources.bio-techne.com/images/products/SHPRH-Antibody-OTI5G8-Azide-and-BSA-Free-Western-Blot-NBP2-74180-img0001.jpg "Western Blot: SHPRH Antibody (OTI5G8)Azide and BSA Free [NBP2-74180]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI5G8
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for SHPRH Antibody (OTI5G8) - Azide and BSA Free
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-100 and 1500-1600 of human SHPRH (NP_775105) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
192.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SHPRH Antibody (OTI5G8) - Azide and BSA Free
Western Blot: SHPRH Antibody (OTI5G8)Azide and BSA Free [NBP2-74180]
Western Blot: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.![Flow Cytometry: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180]](https://resources.bio-techne.com/images/products/SHPRH-Antibody-OTI5G8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74180-img0002.jpg "Flow Cytometry: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180]")
Flow Cytometry: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180]
Flow Cytometry: SHPRH Antibody (OTI5G8) - Azide and BSA Free [NBP2-74180] - Analysis of Jurkat cells, using anti-SHPRH antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for SHPRH Antibody (OTI5G8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SHPRH
Alternate Names
2610103K11Rik, bA545I5.2, E3 ubiquitin-protein ligase SHPRH, EC 3.6.4.-, EC 6.3.2.-, FLJ27258, FLJ37625, FLJ90837, histone-linker, PHD and RING finger domain-containing helicase, KIAA2023FLJ45012, MGC134886, SNF2 histone linker PHD RING helicase
Gene Symbol
SHPRH
Additional SHPRH Products
Product Documents for SHPRH Antibody (OTI5G8) - Azide and BSA Free
Product Specific Notices for SHPRH Antibody (OTI5G8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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