SIAH1/2 Antibody (8G7H12)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-93596
Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine, Drosophila, Mouse (Negative), Zebrafish
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 8G7H12
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]
Reactivity Notes
Does not react with mouse.
Localization
Nuclear, Cytoplasmic
Specificity
This is specific for human SIAH 1 and 2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SIAH1/2 Antibody (8G7H12)
![Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596]](https://resources.bio-techne.com/images/products/SIAH1-2-Antibody-8G7H12-Western-Blot-NB110-93596-img0002.jpg "Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596]")
Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596]
Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speciApplications for SIAH1/2 Antibody (8G7H12)
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SIAH1/2
Alternate Names
E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), siah-1, SIAH1A, siah-1a
Gene Symbol
SIAH1
UniProt
Additional SIAH1/2 Products
Product Documents for SIAH1/2 Antibody (8G7H12)
Product Specific Notices for SIAH1/2 Antibody (8G7H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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