SIAH1 Antibody (2C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006477-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIAH1 Antibodies »

Product Specifications

Immunogen

SIAH1 (NP_003022, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SIAH1 Antibody (2C5)

Western Blot: SIAH1 Antibody (2C5) [H00006477-M02]

Western Blot: SIAH1 Antibody (2C5) [H00006477-M02]

Western Blot: SIAH1 Antibody (2C5) [H00006477-M02] - SIAH1 monoclonal antibody (M02), clone 2C5. Analysis of SIAH1 expression in Jurkat.
Sandwich ELISA: SIAH1 Antibody (2C5) [H00006477-M02] - Detection limit for recombinant GST tagged SIAH1 is 0.03 ng/ml as a capture antibody.

Applications for SIAH1 Antibody (2C5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SIAH1

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Alternate Names

E3 ubiquitin-protein ligase SIAH1, EC 6.3.2, EC 6.3.2.-, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, seven in absentia homolog 1 (Drosophila), Siah-1, SIAH1A, Siah-1a

Gene Symbol

SIAH1

UniProt

NP_003022 (Human)

Additional SIAH1 Products

Product Documents for SIAH1 Antibody (2C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIAH1 Antibody (2C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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