Siglec-1/CD169 Antibody (ED3) [Janelia Fluor® 525]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65301JF525
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Janelia Fluor 525
Antibody Source
Monoclonal Mouse IgG2A Clone # ED3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Rat Spleen cell homogenate
Specificity
NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Siglec-1/CD169 Antibody (ED3) [Janelia Fluor® 525]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Siglec-1/CD169
Long Name
Sialic Acid Binding Ig-like Lectin 1
Alternate Names
CD169, Siglec1
Gene Symbol
SIGLEC1
Additional Siglec-1/CD169 Products
Product Documents for Siglec-1/CD169 Antibody (ED3) [Janelia Fluor® 525]
Product Specific Notices for Siglec-1/CD169 Antibody (ED3) [Janelia Fluor® 525]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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