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Siglec-1/CD169 Antibody (HSn 7D2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08615

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HSn 7D2

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Fc fusion protein containing N-terminal 4 domains of human Siglec-1/CD169. (Uniprot: Q9ZZ2)

Localization

Cell Membrane and Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07624).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Applications for Siglec-1/CD169 Antibody (HSn 7D2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Siglec-1/CD169

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Long Name

Sialic Acid Binding Ig-like Lectin 1

Alternate Names

CD169, Siglec1

Gene Symbol

SIGLEC1

Additional Siglec-1/CD169 Products

Product Documents for Siglec-1/CD169 Antibody (HSn 7D2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Siglec-1/CD169 Antibody (HSn 7D2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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