Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Siglec-11 Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11. Amino Acid Squence: WEPADQEAPSTTEYSEIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Siglec-11 Antibody - BSA Free

Western Blot: Siglec-11 AntibodyBSA Free [NBP1-77025]

Western Blot: Siglec-11 AntibodyBSA Free [NBP1-77025]

Western Blot: Siglec-11 Antibody [NBP1-77025] - HepG2 cell lysate with Siglec11 antibody at 1 ug/mL.
Immunohistochemistry-Paraffin: Siglec-11 Antibody - BSA Free [NBP1-77025]

Immunohistochemistry-Paraffin: Siglec-11 Antibody - BSA Free [NBP1-77025]

Immunohistochemistry-Paraffin: Siglec-11 Antibody [NBP1-77025] - Human liver tissue with Siglec11 antibody at 5 ug/ml.
Siglec-11 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Siglec-11 Antibody - BSA Free [NBP1-77025] -

Immunocytochemistry/ Immunofluorescence: Siglec-11 Antibody - BSA Free [NBP1-77025] - Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 u/mL.

Applications for Siglec-11 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml
Application Notes
Despite its predicted size, Siglec-11 migrates at a higher than expected molecular weight in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-11

Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE.

Long Name

Sialic Acid Binding Ig-like Lectin 11

Alternate Names

Siglec11

Gene Symbol

SIGLEC11

UniProt

NP_443116

Additional Siglec-11 Products

Product Documents for Siglec-11 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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