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Siglec-15 Antibody (3F7F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27165

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-27165-0.025ml
NBP3-27165-0.1ml

Key Product Details

Species Reactivity

Human, Monkey

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F7F3

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human Siglec-15 (AA: Extra(20-263)) expressed in Mammal.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-15 Antibody (3F7F3)

ELISA: Siglec-15 Antibody (3F7F3) [NBP3-27165] -

ELISA: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: Siglec-15 Antibody (3F7F3) [NBP3-27165] -

Western Blot: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis using Siglec-15 mAb against human Siglec-15 (AA: Extra(20-263)) recombinant protein. (Expected MW is 56.4 kDa)

Flow Cytometry: Siglec-15 Antibody (3F7F3) [NBP3-27165] -

Flow Cytometry: Siglec-15 Antibody (3F7F3) [NBP3-27165] - Analysis of Jurkat cells using Siglec-15 mouse mAb (green) and negative control (red).

Applications for Siglec-15 Antibody (3F7F3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-15

SIGLEC15 binds sialylated glycoproteins

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Gene Symbol

SIGLEC15

Additional Siglec-15 Products

Product Documents for Siglec-15 Antibody (3F7F3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Siglec-15 Antibody (3F7F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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