Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54473
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54473-img0003.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]")
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Western-Blot-NBP2-54473-img0006.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BLCAM/1795
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa52-178) of human Siglec-2/CD22 protein (exact sequence is proprietary) (Uniprot: P20273)
Localization
Cell Surface
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53239).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]
Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).![Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54473-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]")
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]
Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54473-img0007.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]")
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Western-Blot-NBP2-54473-img0001.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795)Azide and BSA Free [NBP2-54473]")
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54473-img0002.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]")
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Azide-and-BSA-Free-Protein-Array-NBP2-54473-img0004.jpg "Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free [NBP2-54473]")
Applications for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
Application
Recommended Usage
ELISA
2 - 4 ug/ml
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Siglec-2/CD22
Long Name
Sialic Acid Binding Ig-like Lectin 2
Alternate Names
BL-CAM, CD22, Siglec2
Gene Symbol
CD22
Additional Siglec-2/CD22 Products
Product Documents for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
Product Specific Notices for Siglec-2/CD22 Antibody (BLCAM/1795) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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