SIGLECL12 Antibody (1D1)

Novus Biologicals, part of Bio-Techne | Catalog # H00089858-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Siglec-12 Antibodies »

Product Specifications

Immunogen

SIGLEC12 (NP_443729.1, 503 a.a. ~ 595 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RSCRKKSARPAVGVGDTGMEDANAVRGSASQGPLIESPADDSPPHHAPPALATPSPEEGEIQYASLSFHKARPQYPQEQEAIGYEYSEINIPK

Specificity

Reacts with sialic acid binding Ig-like lectin 12.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SIGLECL12 Antibody (1D1)

Western Blot: SIGLECL12 Antibody (1D1) [H00089858-M01]

Western Blot: SIGLECL12 Antibody (1D1) [H00089858-M01]

Western Blot: SIGLECL12 Antibody (1D1) [H00089858-M01] - Analysis of SIGLEC12 expression in transfected 293T cell line by SIGLEC12 monoclonal antibody (M01), clone 1D1.Lane 1: SIGLEC12 transfected lysate (Predicted MW: 65 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SIGLECL12 Antibody (1D1) [H00089858-M01] - Detection limit for recombinant GST tagged SIGLEC12 is 0.3 ng/ml as a capture antibody.

Applications for SIGLECL12 Antibody (1D1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Siglec-12

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]

Long Name

Sialic Acid Binding Ig-like Lectin 12

Alternate Names

S2V, Siglec-XII, SIGLECL1, SLG

Entrez Gene IDs

89858 (Human)

Gene Symbol

SIGLEC12

UniProt

NP_443729

Additional Siglec-12 Products

Product Documents for SIGLECL12 Antibody (1D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIGLECL12 Antibody (1D1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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