SIKE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82152

Novus Biologicals, part of Bio-Techne
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NBP1-82152
NBP1-82152-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIKE1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPHILLSQENTQIRDLQQENRELWISLEEHQDALEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIKE1 Antibody

Western Blot: SIKE1 Antibody [NBP1-82152]

Western Blot: SIKE1 Antibody [NBP1-82152]

Western Blot: SIKE1 Antibody [NBP1-82152] - Analysis in control (vector only transfected HEK293T lysate) and SIKE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SIKE1 Antibody [NBP1-82152]

Immunocytochemistry/ Immunofluorescence: SIKE1 Antibody [NBP1-82152]

Immunocytochemistry/Immunofluorescence: SIKE1 Antibody [NBP1-82152] - Immunofluorescent staining of human cell line U-251 MG shows localization to focal adhesion sites.
Immunohistochemistry-Paraffin: SIKE1 Antibody [NBP1-82152]

Immunohistochemistry-Paraffin: SIKE1 Antibody [NBP1-82152]

Immunohistochemistry-Paraffin: SIKE1 Antibody [NBP1-82152] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for SIKE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIKE1

SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029)and virus-triggered interferon activation pathways (Huang et al., 2005 (PubMed 16281057)).(supplied by OMIM)

Alternate Names

DKFZp686A0768, SIKEFLJ21168, suppressor of IKBKE 1, suppressor of IKK epsilon, Suppressor of IKK-epsilon

Gene Symbol

SIKE1

Additional SIKE1 Products

Product Documents for SIKE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIKE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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