SIN3A Antibody (8V5B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16124

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16124-100ul
NBP3-16124-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V5B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SIN3A Antibodies »

Product Summary for SIN3A Antibody (8V5B6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human SIN3A (Q96ST3). YKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIN3A Antibody (8V5B6)

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124]

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124]

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124] - Western blot analysis of extracts of various cell lines, using SIN3A antibody (NBP3-16124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124]

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunofluorescence analysis of U-2 OS cells using SIN3A Rabbit mAb (NBP3-16124) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SIN3A Antibody (8V5B6) [NBP3-16124]

Immunohistochemistry-Paraffin: SIN3A Antibody (8V5B6) [NBP3-16124]

Immunohistochemistry-Paraffin: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry of paraffin-embedded mouse brain using SIN3A Rabbit mAb (NBP3-16124) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for SIN3A Antibody (8V5B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIN3A

Sin3A is encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Long Name

SIN3 Homolog A

Alternate Names

DKFZP434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A

Gene Symbol

SIN3A

Additional SIN3A Products

Product Documents for SIN3A Antibody (8V5B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIN3A Antibody (8V5B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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