SIN3A Antibody

Chromatin Immunoprecipitation: SIN3A Antibody [NB600-1263] - Analysis of Sin3A performed using cross-linked chromatin from colon carcinoma cells treated with serum. Immunoprecipitation performed using a multiplex microplate Matrix ChIP assay with Sin3A polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. A representation of the Egr-1 locus is shown; boxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263]

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).