SIPA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98740

Novus Biologicals, part of Bio-Techne
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NBP2-98740-100ul
NBP2-98740-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SIPA1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SIPA1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SIPA1 Antibody

Western Blot: SIPA1 Antibody [NBP2-98740]

Western Blot: SIPA1 Antibody [NBP2-98740]

Western Blot: SIPA1 Antibody [NBP2-98740] - Anti-SIPA1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 112 kDa. Observed band size: 115 kDa
Immunocytochemistry/ Immunofluorescence: SIPA1 Antibody [NBP2-98740]

Immunocytochemistry/ Immunofluorescence: SIPA1 Antibody [NBP2-98740]

Immunocytochemistry/Immunofluorescence: SIPA1 Antibody [NBP2-98740] - Immunofluorescence staining of SIPA1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human SIPA1 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-98740]

Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-98740]

Immunohistochemistry-Paraffin: SIPA1 Antibody [NBP2-98740] - Immunochemical staining of human SIPA1 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).

Applications for SIPA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:250-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIPA1

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq]

Alternate Names

GTPase-activating protein Spa-1, MGC102688, MGC17037, p130 SPA-1, signal-induced proliferation-associated 1, signal-induced proliferation-associated protein 1, Sipa-1, SPA1signal-induced proliferation-associated gene 1

Gene Symbol

SIPA1

Additional SIPA1 Products

Product Documents for SIPA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIPA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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