SIRT4 Antibody (1C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00023409-M03

Novus Biologicals, part of Bio-Techne
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H00023409-M03
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 4/SIRT4 Antibodies »

Product Specifications

Immunogen

SIRT4 (NP_036372.1, 215 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FQVPTCVQCGGHLKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILNIGPTRSDDLACLKLNSRCGELLPLIDPC

Specificity

SIRT4 - sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) (1C8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SIRT4 Antibody (1C8)

Western Blot: SIRT4 Antibody (1C8) [H00023409-M03]

Western Blot: SIRT4 Antibody (1C8) [H00023409-M03]

Western Blot: SIRT4 Antibody (1C8) [H00023409-M03] - Analysis of SIRT4 expression in Raw 264.7.
ELISA: SIRT4 Antibody (1C8) [H00023409-M03]

ELISA: SIRT4 Antibody (1C8) [H00023409-M03]

ELISA: SIRT4 Antibody (1C8) [H00023409-M03] - Detection limit for recombinant GST tagged SIRT4 is 1 ng/ml as a capture antibody.

Applications for SIRT4 Antibody (1C8)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using H00023409-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 4/SIRT4

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq]

Long Name

Silent Mating Type Information Regulation 4 Homolog

Alternate Names

SIR2L4, SIRT4

Entrez Gene IDs

23409 (Human)

Gene Symbol

SIRT4

UniProt

NP_036372 (Human)

Additional Sirtuin 4/SIRT4 Products

Product Documents for SIRT4 Antibody (1C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRT4 Antibody (1C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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