SIRT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1406

Novus Biologicals, part of Bio-Techne
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NB100-1406
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Sirtuin 4/SIRT4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SRCGELLPLIDPC corresponding to C-Terminus according to NP_036372.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SIRT4 Antibody

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SIRT4 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/mL

Immunohistochemistry-Paraffin

2 - 3 ug/mL

Peptide ELISA

Detection limit 1:3200

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 37 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa band according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human pancreas shows staining of the islets of langerhans.
IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 4/SIRT4

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Long Name

Silent Mating Type Information Regulation 4 Homolog

Alternate Names

SIR2L4, SIRT4

Entrez Gene IDs

23409 (Human); 75387 (Mouse)

Gene Symbol

SIRT4

UniProt

NP_036372. (Human)

Additional Sirtuin 4/SIRT4 Products

Product Documents for SIRT4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SIRT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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