SIRT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1406

Novus Biologicals, part of Bio-Techne
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NB100-1406
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Sirtuin 4/SIRT4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence SRCGELLPLIDPC corresponding to C-Terminus according to NP_036372.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SIRT4 Antibody

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406]

Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SIRT4 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/mL

Immunohistochemistry-Paraffin

2 - 3 ug/mL

Peptide ELISA

Detection limit 1:3200

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 37 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa band according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human pancreas shows staining of the islets of langerhans.
IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 4/SIRT4

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Long Name

Silent Mating Type Information Regulation 4 Homolog

Alternate Names

SIR2L4, SIRT4

Entrez Gene IDs

23409 (Human); 75387 (Mouse)

Gene Symbol

SIRT4

UniProt

NP_036372. (Human)

Additional Sirtuin 4/SIRT4 Products

Product Documents for SIRT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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