SIRT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80746

Novus Biologicals, part of Bio-Techne
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NBP1-80746
NBP1-80746-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 4/SIRT4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PCSKASIGLFVPASPPLDPEKVKELQRFITLSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIRT4 Antibody

Western Blot: SIRT4 Antibody [NBP1-80746]

Western Blot: SIRT4 Antibody [NBP1-80746]

Western Blot: SIRT4 Antibody [NBP1-80746] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining in human testis and pancreas tissues using anti-SIRT4 antibody. Corresponding SIRT4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746]

Immunohistochemistry-Paraffin: SIRT4 Antibody [NBP1-80746] - Staining of human pancreas shows low expression as expected.
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Applications for SIRT4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 4/SIRT4

SIRT4 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Long Name

Silent Mating Type Information Regulation 4 Homolog

Alternate Names

SIR2L4, SIRT4

Entrez Gene IDs

23409 (Human)

Gene Symbol

SIRT4

UniProt

Q9Y6E7 (Human)

Additional Sirtuin 4/SIRT4 Products

Product Documents for SIRT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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