SIRT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98856

Novus Biologicals, part of Bio-Techne
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NBP2-98856-100ul
NBP2-98856-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 4/SIRT4 Antibodies »

Product Summary for SIRT4 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SIRT4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SIRT4 Antibody

Western Blot: SIRT4 Antibody [NBP2-98856]

Western Blot: SIRT4 Antibody [NBP2-98856]

Western Blot: SIRT4 Antibody [NBP2-98856] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa
Immunoprecipitation: SIRT4 Antibody [NBP2-98856]

Immunoprecipitation: SIRT4 Antibody [NBP2-98856]

Immunoprecipitation: SIRT4 Antibody [NBP2-98856] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-SIRT4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-SIRT4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa

Applications for SIRT4 Antibody

Application
Recommended Usage

Immunoprecipitation

0.1-0.5 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 4/SIRT4

SIRT4 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Long Name

Silent Mating Type Information Regulation 4 Homolog

Alternate Names

SIR2L4, SIRT4

Gene Symbol

SIRT4

Additional Sirtuin 4/SIRT4 Products

Product Documents for SIRT4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SIRT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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