Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SIRT6 Antibodies »

Product Summary for SIRT6 Antibody (OTI1G3) - Azide and BSA Free

Immunogen

Human recombinant protein fragment corresponding to amino acids 88-352 of human SIRT6(NP_057623) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIRT6 Antibody (OTI1G3) - Azide and BSA Free

Western Blot: SIRT6 Antibody (OTI1G3)Azide and BSA Free [NBP2-74198]

Western Blot: SIRT6 Antibody (OTI1G3)Azide and BSA Free [NBP2-74198]

Western Blot: SIRT6 Antibody (OTI1G3) - Azide and BSA Free [NBP2-74198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT6.
Immunocytochemistry/ Immunofluorescence: SIRT6 Antibody (OTI1G3) - Azide and BSA Free [NBP2-74198]

Immunocytochemistry/ Immunofluorescence: SIRT6 Antibody (OTI1G3) - Azide and BSA Free [NBP2-74198]

Immunocytochemistry/Immunofluorescence: SIRT6 Antibody (OTI1G3) - Azide and BSA Free [NBP2-74198] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6.

Applications for SIRT6 Antibody (OTI1G3) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIRT6

Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6)--a mouse Sir2 homolog--is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in the ageing process.

Long Name

NAD-dependent protein deacylase sirtuin-6

Alternate Names

EC 2.3.1.286, hSIRT6, SIR2-like protein 6, SIR2L6

Gene Symbol

SIRT6

Additional SIRT6 Products

Product Documents for SIRT6 Antibody (OTI1G3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRT6 Antibody (OTI1G3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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