Sirtuin 3/SIRT3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87037
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Sirtuin 3/SIRT3 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYT
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sirtuin 3/SIRT3 Antibody
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-87037]](https://resources.bio-techne.com/images/products/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP1-87037-img0007.jpg "Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-87037]")
Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-87037]
Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-87037] - Analysis in control (vector only transfected HEK293T lysate) and SIRT3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037]](https://resources.bio-techne.com/images/products/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP1-87037-img0006.jpg "Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037]")
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037]
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-87037] - Staining of human skin shows strong cytoplasmic positivity in the basal layer of epidermal cells.Applications for Sirtuin 3/SIRT3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Sirtuin 3/SIRT3
Long Name
Silent Mating Type Information Regulation 3 Homolog
Alternate Names
SIR2-like protein 3, SIR2L3, SIRT3
Entrez Gene IDs
23410 (Human)
Gene Symbol
SIRT3
UniProt
Additional Sirtuin 3/SIRT3 Products
Product Documents for Sirtuin 3/SIRT3 Antibody
Product Specific Notices for Sirtuin 3/SIRT3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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