SIT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52153

Novus Biologicals, part of Bio-Techne
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NBP1-52153
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SIT1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HLSQWTRGRSRSH corresponding to internal region according to NP_055265.1.

Epitope

C-HLSQWTRGRSRSH

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Applications for SIT1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000
Application Notes
WB: Preliminary experiments in human lymph node, spleen, tonsil and thymus lysates gave no specific signal but low background.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIT1

SIT is a nbsp40 kD disulfide-linked protein also known as SHP2-interacting transmembrane adaptor protein. SIT is a membrane protein containing a very short extracellular domain (18 amino acids). SIT is expressed in T cells, but is not expressed in monocytes. SIT is a signaling adaptor molecule that negatively regulates T cell activation through SHP-2 interactions. The SIT protein is phosphorylated by Src kinases and has been shown to associate with SHP2, Csk, Zap70, Syk, Fyn, Lck, and Grb2. SIT is also heavily glycosylated and treatment with endoglycosidase has been shown to reduce the apprarent molecular weight to approximately 20 kD. The SIT-40 antibody recognizes the intracellular region of human SIT and has been shown to be useful for Western blotting.

Long Name

Signaling Threshold Regulating Transmembrane Adaptor 1

Alternate Names

gp30/40

Entrez Gene IDs

27240 (Human)

Gene Symbol

SIT1

UniProt

NP_055265.1 (Human)

Additional SIT1 Products

Product Documents for SIT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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