Skp1 Antibody (S01-6H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14975

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14975-100ul
NBP3-14975-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-6H2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SKP1 Antibodies »

Product Summary for Skp1 Antibody (S01-6H2)

Immunogen

A synthetic peptide of human Skp1 (Uniprot#: P63208)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Skp1 Antibody (S01-6H2)

Western Blot: Skp1 Antibody (S01-6H2) [NBP3-14975]

Western Blot: Skp1 Antibody (S01-6H2) [NBP3-14975]

Western Blot: Skp1 Antibody (S01-6H2) [NBP3-14975] - Western blot detection of Skp1 in K562, Rat Brain, C6, 3T3 cell lysates using Skp1 Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (S01-6H2) [NBP3-14975]

Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (S01-6H2) [NBP3-14975]

Immunocytochemistry/Immunofluorescence: Skp1 Antibody (S01-6H2) [NBP3-14975] - Immunocytochemistry of Skp1 (green) in MCF-7 using Skp1 antibody at dilution 1/20, and DAPI (blue)

Applications for Skp1 Antibody (S01-6H2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000-1/5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKP1

Skp1 encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Long Name

S-phase Kinase Associated Protein 1

Alternate Names

OCP2, p19a, p19Skp1, SKP1A

Gene Symbol

SKP1

Additional SKP1 Products

Product Documents for Skp1 Antibody (S01-6H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Skp1 Antibody (S01-6H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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