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SLC6A12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88641

Novus Biologicals, part of Bio-Techne
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NBP1-88641
NBP1-88641-25ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLC6A12 Antibody

Western Blot: SLC6A12 Antibody [NBP1-88641]

Western Blot: SLC6A12 Antibody [NBP1-88641]

Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: SLC6A12 Antibody [NBP1-88641]

Immunocytochemistry/ Immunofluorescence: SLC6A12 Antibody [NBP1-88641]

Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Immunofluorescent cytostaining. Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFNgamma and 30 ng/ml TNFalpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fneur.2018.00846/full), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641]

Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641]

Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.

Applications for SLC6A12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC6A12

Transports betaine and GABA. May have a role in regulation of GABAergic transmission in the brain through the reuptake of GABA into presynaptic terminals, as well as in osmotic regulation

Long Name

Sodium- and chloride-dependent betaine transporter

Alternate Names

BGT-1

Gene Symbol

SLC6A12

Additional SLC6A12 Products

Product Documents for SLC6A12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SLC6A12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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