SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25549
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2353
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SLC7A5/LAT1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] -
Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] -
Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] -
Western Blot: SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free [NBP3-25549] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (NBP3-25549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC7A5/LAT1
Long Name
Solute Carrier Family 7 Member 5
Alternate Names
4F2LC, CD98lc, E16, LAT1, MPE16, TA1
Gene Symbol
SLC7A5
Additional SLC7A5/LAT1 Products
Product Documents for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
Product Specific Notices for SLC7A5/LAT1 Antibody (HL2353) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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