SLC8A3/NCX3 Antibody (C2C12)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2869

Novus Biologicals, part of Bio-Techne
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NB120-2869
NB120-2869-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Insect, Kangaroo, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # C2C12

Concentration

1 mg/ml

View all SLC8A3/NCX3 Antibodies »

Product Specifications

Immunogen

Purified canine cardiac sodium/calcium exchanger.

Epitope

This antibody recognizes an epitope between amino acids 371-525 on the intracellular side of the plasma membrane.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SLC8A3/NCX3 Antibody (C2C12)

Immunocytochemistry/ Immunofluorescence: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunocytochemistry/ Immunofluorescence: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. U251 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.
Immunohistochemistry-Paraffin: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunohistochemistry-Paraffin: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunohistochemistry-Paraffin: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.
Immunocytochemistry/ Immunofluorescence: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunocytochemistry/ Immunofluorescence: SLC8A3/NCX3 Antibody (C2C12) [NB120-2869]

Immunocytochemistry/Immunofluorescence: Sodium-Calcium Exchanger Antibody (C2C12) [NB120-2869] - Sodium/Calcium Exchanger staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. A2058 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Calcium Exchanger at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary.
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Applications for SLC8A3/NCX3 Antibody (C2C12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000
Application Notes
ELISA usage was reported in the scientific literature (PMID: 7692739). WB: Detects an approx. 120 kDa protein representing the sodium / calcium exchanger from guinea pig cardiac extract. Also detects 70 kDa and 160 kDa proteins representing a proteolytic fragment and non-reduced exchanger respectively. T-tubular membrane.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS +BSA(1mg/ml)

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC8A3/NCX3

The sodium/calcium exchanger of cardiac sarcolemma rapidly transports calcium during excitation-contraction coupling and is the dominant myocardial calcium efflux mechanism. The sodium/calcium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium against its electrochemical gradient in a 3 sodium : 1 calcium exchange. Sodium/calcium exchange activity is present in excitable cells and in non-excitable cells.

Long Name

Solute Carrier Family 8 Member A3

Alternate Names

NCX3, SLC8A3

Gene Symbol

SLC8A3

Additional SLC8A3/NCX3 Products

Product Documents for SLC8A3/NCX3 Antibody (C2C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SLC8A3/NCX3 Antibody (C2C12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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