SLFN5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81178

Novus Biologicals, part of Bio-Techne
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NBP1-81178
NBP1-81178-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SLFN5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ERHGVGLDVPPIFRSHLDKMQKENHFLIFVKSWNTEAGVPLATLCSNLYHRERTSTDVMDSQEALAFLKCRTQTPTNINVSNSLGPQAAQGSVQYEGNINVSAAALFDRKRLQYLEKLNLPESTHVEFVMFSTDVSHCVKD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLFN5 Antibody

Western Blot: SLFN5 Antibody [NBP1-81178]

Western Blot: SLFN5 Antibody [NBP1-81178]

Western Blot: SLFN5 Antibody [NBP1-81178] - Analysis in human cell lines SK-MEL-30 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178]

Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178]

Immunocytochemistry/Immunofluorescence: SLFN5 Antibody [NBP1-81178] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]

Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178]

Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes as expected.
View 5 more images

Applications for SLFN5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLFN5

SLFN5 may have a role in hematopoeitic cell differentiation

Alternate Names

MGC150611, MGC150612, MGC19764, schlafen family member 5

Entrez Gene IDs

162394 (Human)

Gene Symbol

SLFN5

Additional SLFN5 Products

Product Documents for SLFN5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SLFN5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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