SLIRP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-37258

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB110-37258SS
NB110-37258
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SLIRP Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]

Localization

Cytoplasm, Mitochondria.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLIRP Antibody - BSA Free

Western Blot: SLIRP AntibodyBSA Free [NB110-37258]

Western Blot: SLIRP AntibodyBSA Free [NB110-37258]

Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.
Immunocytochemistry/ Immunofluorescence: SLIRP Antibody - BSA Free [NB110-37258]

Immunocytochemistry/ Immunofluorescence: SLIRP Antibody - BSA Free [NB110-37258]

Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SLIRP (NB110-37258) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: SLIRP Antibody - BSA Free [NB110-37258]

Immunohistochemistry: SLIRP Antibody - BSA Free [NB110-37258]

Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.
View 2 more images

Applications for SLIRP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Simple Western

10 ug/ml

Western Blot

0.5-2.0 ug/ml
Application Notes
In Western Blot, a band is seen approx. 12 kDa representing the SLIRP protein. May see additional bands above 45 kDa. In ICC/IF, staining of the mitochondria was observed in U2OS cells. In IHC-P, cytoplasmic and mitochondrial staining was observed in mouse intestine tissue.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLIRP

SLIRP (SRA stem-loop-interacting RNA-binding protein) is RNA binding protein found predominantly in mitochondria and show some nuclear expression also where it is recruited to nuclear receptor target promoters. It is ubiquitously expressed with highest expression levels in heart, liver, skeletal muscle, testis etc. and has been reported in cancers also. SLIRP was initially identified in a yeast three-hybrid screen via its binding to STR7 loop of SRA RNA (stem-loop structure 7 of steroid receptor RNA activator), RNA activator that mediates activities of estrogen receptor. Inside the nucleus, SLIRP is recruited to estrogen-responsive promoters for repressing SRA-mediated coactivation. However, it is localized mainly in mitochondria, and disruption of mitochondrial localization sequence at its N-terminus diminishes its ability to repress transcription inside nucleus, suggesting that mitochondrial localization of SLIRP is crucial to its nuclear corepressor activity. Inside mitochondira, SLIRP is present in a large ribonucleoprotein complex and maintains mitochondrial mRNA homeostasis by regulating mRNA decay as well as polyadenylation. SLIRP also represses AR, TR (thyroid), GR (glucocorticoid) and VDR-mediated transactivation.

Alternate Names

chromosome 14 open reading frame 156, DC50, PD04872, SLIRP, SRA stem-loop-interacting RNA-binding protein, mitochondrial

Gene Symbol

SLIRP

Additional SLIRP Products

Product Documents for SLIRP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SLIRP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.